Review Article
Bacteriophages and Their Immunological Applications against Infectious Threats
Table 2
Biofilm control by EPS depolymerase studies in vitro.
| Target pathogen | Observations | Concerns | References |
| Pseudomonas putida | Biofilm clearance: significant variations between bacterial strains upon biofilm aging | Reduction of aged biofilm susceptibility to phage infection because of their thickness and phenotypic changes | [74] | Klebsiella pneumoniae | Greater biofilm clearance if cotreated with ciprofloxacin | Possible inhibition of depolymerase activity by ciprofloxacin | [75] | Escherichia coli | Depolymerase-producing phage construct capable of biofilm clearance | Results obtained for an engineered T7 strain | [76] | Enterobacter cloace | Biofilm clearance with depolymerase-producing phage; enhancement of chemical antibacterial penetration after phage-free depolymerase treatment | Combinations of three phages required for eradication of single-species biofilms; ineffective treatment of dual-species biofilms | [77] | Pseudomonas aeruginosa | Old biofilm clearance (20 days) | Bacteriophage migration facilitated by reduction of alginate viscosity | [78] | Enterobacter agglomerans and Serratia marcescens | Phage-resistant bacteria biofilm clearance with treatment of phage or phage-free depolymerase | Little differences in the chemical composition of EPS prevent the degradation of the polymer | [79] | Enterobacter agglomerans | Dual-species biofilm clearance with phage-free depolymerase | Specific depolymerase: no degradation of single biofilms of Klebsiella pneumoniae’s EPS | [80] |
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