Journal of Immunology Research / 2017 / Article / Fig 1

Research Article

Regulation of Discrete Functional Responses by Syk and Src Family Tyrosine Kinases in Human Neutrophils

Figure 1

Expression and cellular distribution of Src family and Syk tyrosine kinases in human neutrophils. (a) Neutrophils (pmn) were disrupted by nitrogen cavitation; cytoplasmic and nuclear fractions were then processed for immunoblot analysis of Src family and Syk kinases (0.5 × 106 cell equivalents were loaded per lane). (b) Neutrophil subcellular fractions prepared as above were processed for immunoblot analysis of the cytosolic marker leukotriene A4 hydrolase (LTAH) or the nuclear marker histone H3 (HH3). The experiment shown in this figure is a representative of three.
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