Regulation of Discrete Functional Responses by Syk and Src Family Tyrosine Kinases in Human Neutrophils
Effect of STK and Syk inhibition on delayed apoptosis in human neutrophils. Cells were pretreated with STK or Syk inhibitors, prior to a 20 h culture with LPS, TNFα, fMLP, or GM-CSF, as described for Figure 2. Cells were then processed for FACS analysis of annexin V binding; a minimum of 10,000 cells were processed for each sample. Results are expressed as mean ± SEM of at least 3 independent experiments. §, versus unstimulated cells (“ctrl”); versus matched condition without inhibitor.