Journal of Immunology Research / 2017 / Article / Fig 5

Research Article

Regulation of Discrete Functional Responses by Syk and Src Family Tyrosine Kinases in Human Neutrophils

Figure 5

Effect of STK and Syk inhibition on delayed apoptosis in human neutrophils. Cells were pretreated with STK or Syk inhibitors, prior to a 20 h culture with LPS, TNFα, fMLP, or GM-CSF, as described for Figure 2. Cells were then processed for FACS analysis of annexin V binding; a minimum of 10,000 cells were processed for each sample. Results are expressed as mean ± SEM of at least 3 independent experiments. §, versus unstimulated cells (“ctrl”); versus matched condition without inhibitor.