Synergistic Antitumour Properties of viscumTT in Alveolar Rhabdomyosarcoma
viscumTT induces apoptosis synergistically ex vivo. Ex vivo cultures from three different patients were incubated with viscum, TT, and viscumTT in increasing concentrations for 48 h (a) patient no. 1 or for 24 h, (b) patient no. 2, and (c) patient no. 3. Cell proliferation was measured by CASY cell counter analysis, and apoptosis was assessed by annexin V/PI assay and flow cytometry. All results are presented as means ± SD of two independent experiments. Webb’s fractional product (Fp) was calculated to assess synergism, values Fp > 1 display synergism. Mistletoe lectin (ML) and oleanolic acid (OA) concentrations were used as marker substances for viscum and TT, respectively.
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