Adenosine Triphosphate Promotes Allergen-Induced Airway Inflammation and Th17 Cell Polarization in Neutrophilic Asthma

Figure 7

Suramin inhibits the Th17 response of ATP-γS and OVA_323–339 peptide-stimulated splenocytes from DO11.10 mice in vitro. (a) Suramin inhibited production of IL-17A in ATP-γS and OVA_323–339 peptide-stimulated splenocytes. The splenocytes from DO11.10 mice were cultured in medium only, stimulated with OVA_323–339 peptide (50 mmol/L) or (and) ATP-γS (100 μM), or treated with graded concentrations of suramin (100, 200, and 400 μM) for 5 days. IL-17A levels in the supernatants were measured using ELISAs. The values represent the means ± SEM (). compared with the ATP-γS and OVA_323–339 peptide-stimulated splenocyte group. (b, c) Suramin inhibited Th17 polarization induced by ATP-γS and OVA_323–339 peptide-stimulated splenocytes. The percentages of IL-17⁺ CD4⁺ T cells were analyzed by flow cytometry (b). (c) Histogram showing the percentages of IL-17⁺ CD4⁺ T cells after 5 days of treatment. Values represent the means ± SEM (). compared with the ATP-γS and OVA_323–339 peptide-stimulated splenocyte group.

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