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Journal of Immunology Research
Volume 2017, Article ID 5871043, 10 pages
https://doi.org/10.1155/2017/5871043
Research Article

Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis

1Basic Pathology Department, Federal University of Paraná, Curitiba, PR, Brazil
2Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, Brazil

Correspondence should be addressed to Vanete Thomaz Soccol; moc.liamg@loccosetenav

Received 26 September 2016; Revised 20 November 2016; Accepted 8 December 2016; Published 7 March 2017

Academic Editor: Kristen M. Kahle

Copyright © 2017 Juliana Seger Link et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This work’s goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund’s adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L. braziliensis. Sera from hamsters which had peptides P2 and P3, as well as the MIX, administered presented antibodies that recognized proteins between 52 and 76 kDa of L. braziliensis. The research on the similarity of the peptides’ sequences in protein databases showed that they match a 63 kDa glycoprotein. The three peptides and the MIX were recognized by the sera from CL patients by immunoassay approach (ELISA). The peptides’ MIX showed the best performance (79% sensitivity) followed by the P1 (72% sensitivity), and the AS presented 91% sensitivity. These results show a new route for discovering molecules for diagnosis or for immunoprotection against leishmaniosis.