Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis

Figure 1

Reactivity of peptides with sera from immunized hamsters by ELISA. P1 peptide (a), P2 peptide (b), P3 peptide (c), and MIX of P1, P2, and P3 (d) were tested with anti-peptide serum P1 (P1), anti-peptide serum P2 (P2), anti-peptide serum P3 (P3), anti-peptide serum MIX (MIX), nonimmune serum, and serum against Freund’s adjuvant. ELISA plates were coated with peptide (2 μg/mL) and incubated with hamster serum diluted at 1 : 50 (pool of sera with ten animals in each group). The detection reaction was conducted with anti-hamster IgG (whole molecule) peroxidase antibody (200 ng/mL).

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