Research Article

Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis

Figure 2

Anti-peptide antibody reactivity to L. braziliensis antigens by western blot. Forty micrograms of L. braziliensis antigen was separated by 15% SDS-PAGE and then, using western blot, was reacted with anti-peptide serum P1 (lane 1), anti-peptide serum P2 (lane 2), anti-peptide serum P3, anti-peptide serum MIX, sera against Freund’s adjuvant (lane 5), and NC = negative control → nonimmune serum (lane 6). Sera from the immunized hamster were tested at a 1 : 100 dilution. The detection reaction was performed with anti-hamster IgG (whole molecule) antibody conjugated to peroxidase (100 μg/mL).