ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study

Table 1

Summary of the main sample characteristics of the samples included in the study.

	Sample 1	Sample 2	Sample 3
Disease	Crohn’s disease (Remicade therapy)	Unknown	Sjögren’s syndrome
Diagnosis/follow-up	Follow-up	Diagnosis	Unknown
Origin	Diluted native OLV patient sample	Diluted native GZA and OLV patient sample	Native patient sample, used by WIV (EQA SN/11641)
Identified autoantibodies	None	CENP-B	SSA/Ro60 + SSB/La
Indirect immunofluorescence pattern on HEp-2	Homogeneous	Centromere	Speckled

Diagnosis/Follow-up = at time of diagnosis versus follow-up of known diagnosis; OLV = Onze Lieve Vrouw Hospital Aalst; GZA = GasthuisZusters Hospital Antwerp; WIV = Scientific Institute of Public Health Brussels; EQA = extern quality control; SN = sample number. Tested for dsDNA, nRNP/Sm, Sm, SSA/Ro60, Ro52, SSB/La, Scl-70, Jo-1, PCNA, PM-Scl, Rib-P, and CENP-B IgG.