Research Article

Th1/Th2 Balance and Th17/Treg-Mediated Immunity in relation to Murine Resistance to Dextran Sulfate-Induced Colitis

Figure 5

A differential induction of Th17 and Treg responses in BALB/c and C57BL/6 mice following DSS challenge. (a) mRNA expression of various cytokines in LPLs and MLNs was analyzed by RT-PCR. Levels of mRNA were normalized to β-actin mRNA and expressed relative to unchallenged BALB/c mice. Data indicate the mean ± SD obtained from a representative of two independent experiments (each with ). (b), (c) LPLs (MLN) were stained for cytokines and key nuclear immune factors and analyzed by FACS. Representative plots (left panel) and associated quantification displaying the mean ± SD values of the percentage of Th17 and Treg cells (right panel) are shown ( mice per group). The data are representative of two independent experiments. Statistical differences were evaluated by Student’s t-test. ; . In all assays, the physical (day 0) cell number of immune cell subtypes is 200,000/sample.
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