Therapeutic Effects of Methanol Extract from Euphorbia kansui Radix on Imiquimod-Induced Psoriasis

Figure 5

Effects of oral administration of EKC in axillary lymph nodes and brachial lymph nodes of psoriasis-induced mice. (a) At the end of the experimental day, mice were sacrificed and the axillary lymph node was isolated. The axillary lymph node cells were stimulated with anti-CD3 Ab for 2 days, and the supernatant was harvested. The cytokine levels were measured by ELISA. Values represent the mean ± SD. (b) The axillary lymph node cells were stimulated with plate-bound anti-CD3 Ab for 2 days. Total mRNA was extracted, and the levels of IL-17A, IL-22, and RORγt were measured by real-time PCR. Values represent the median. (c) At the end of the experimental day, mice were sacrificed and the axillary lymph node was isolated. The axillary lymph node cells were stimulated with anti-CD3 Ab for 2 days, and the supernatant was harvested. Cytokine levels were measured by ELISA. (d) The brachial lymph node cells were stimulated with plate-bound anti-CD3 Ab for 2 days. Total mRNA was extracted, and the levels of IL-17A, IL-22, and RORγt were measured by real-time PCR. Values represent the median. ; ; and compared with the imiquimod + water group.

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