Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response

Figure 7

ECF suppresses IFN-γ-related pathway in TCR engagement-mediated T lymphocyte activation. Purified CD4⁺ T cells were stimulated with anti-CD3 (5 μg/ml) and anti-CD28 (2 μg/ml) for 16 h. Total RNA was isolated using RNeasy kits, and 1 μg of total RNA was used to synthesis cDNA. Real-time quantitative PCR assay was carried out using SYBR Premix Ex Taq II kit, and relative quantification of mRNA expression was calculated as the fold increase using the delta-delta Ct; the housekeeping gene is β-actin. Results presented are mean ± s.e.m., . , versus vehicle group.

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