Dietary Supplementation of Astragalus Polysaccharides Enhanced Immune Components and Growth Factors EGF and IGF-1 in Sow Colostrum

Tan, Lunbo; Wei, Ting; Yuan, Anwen; He, Jun; Liu, Jinhui; Xu, Daojun; Yang, Qing

doi:https://doi.org/10.1155/2017/9253208

Journal of Immunology Research

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Volume 2017 | Article ID 9253208 | https://doi.org/10.1155/2017/9253208

Dietary Supplementation of Astragalus Polysaccharides Enhanced Immune Components and Growth Factors EGF and IGF-1 in Sow Colostrum

Lunbo Tan,¹Ting Wei,¹Anwen Yuan,¹Jun He,²Jinhui Liu,¹Daojun Xu,¹and Qing Yang¹

Academic Editor: Menaka C. Thounaojam

Received02 Aug 2016

Accepted13 Dec 2016

Published09 Jan 2017

Abstract

Colostrum is the main external resource providing piglets with nutrients and maternal immune molecules. Astragalus polysaccharides (APS) have been used as immunopotentiators in vitro and several animal models. This study aimed to determine the effects of APS on immune factors in sow colostrum and milk. The sow diet was supplemented with APS one week before the expected delivery date. Colostrum and milk were collected and designated as 0 h- (onset of parturition), 12 h-, and 24 h-colostrum and 36 h-milk postpartum. Samples were measured using porcine immunoglobulin (Ig) G, IgM, classical swine fever virus antibody (CSFV Ab), epidermal growth factor (EGF), and insulin-like growth factor- (IGF-) 1 ELISA Quantitation Kits. Dietary supplementation of APS significantly enhanced the presence of IgG, IgM, EGF, and IGF-1 in 0 h-colostrum (). The blocking rates of CSFV Ab were increased in samples from APS-supplemented sow when compared to those from the matched samples without APS treatment. The results indicate that supplement of APS could improve the immune components in sow colostrum and/or milk; and status of some specific vaccination could be determined through using colostrum or early milk in sow.

1. Introduction

Newborn piglets can hardly obtain passive immunity from maternal blood during fetal period because of the special epitheliochorial structure of pig placenta. Before their own immune system is fully developed, colostrum is the sole external resource which provides piglets with nutrients, maternal immune molecules, and growth factors [1, 2]. Colostrum production lasts for 24 h after the onset of parturition in swine; afterwards, breast secretion is called milk [3]. The maternal molecules include nonspecific immunoglobulins like immunoglobulin (Ig) G, IgM, and IgA as well some specific antibodies [4, 5]. Maternal blood antibodies in colostrum are transferred to newborn piglets to supply protection against foreign antigens. Piglets have the best maternal immunoglobulin absorption from 4 h to 24 h postpartum, and during this period IgG and IgM are principal immunoglobulins in colostrum; after three days delivery, IgA is the primary immunoglobulin in milk [4, 6]. Factors in colostrum play important roles in promoting the development of the gastrointestinal tract of piglets [5]. Studies indicate that the volume of colostrum intake by piglets is highly related to their health and growth [7, 8].

Astragalus polysaccharides (APS) isolated from a traditional Chinese medicinal herb Astragalus mongholicus are potentially used as immunopotentiators which could increase serum antibody titer and enhance secretion of a wide range of cytokines [9–13]. Supplementation of APS could increase the immunostimulatory effects against several animal viruses like H9N2 avian influenza virus, foot and mouth disease virus, Newcastle disease virus, and infectious bursal disease virus [9, 13, 14]. Diarrhea and dyspepsia are common diseases for piglets due to the immature digestive system. Studies indicated that growth factors epidermal growth factor (EGF) and insulin-like growth factor-1 (IGF-1) in colostrum and milk play important roles in piglet intestinal growth and development [15]. In weaned pigs, optimal dietary APS has beneficial effect on piglet growth performance and immune function [16].

To study the effects of APS on immune function in sow colostrum, dietary APS supplementation was administrated prior to one week of parturition; concentrations of nonspecific immune factors IgG and IgM were measured as well as titer of the specific antibody against the classical swine fever virus (CSFV) after vaccination. Levels of growth factors including EGF and IGF-1 were also quantified.

2. Materials and Methods

2.1. Animals

Twenty crossbred sows (large white × landrace) with same number of parturitions were used from a commercial herd, Tianzhao Garden Animal Husbandry Co. Ltd. (Yueyang City, Hunan Province, China). All sows were vaccinated with a swine fever vaccine (Qianyuanhao Biology Co., Ltd., Beijing, China) on day 25 following the previous parturition. One week prior to the expected date of delivery, all pregnant sows were transferred to individual farrowing crates and randomly separated into two groups as the control group () and APS group (). The control group was fed a common control diet (Table 1). The APS group received the same diet supplemented with APS powder (1.5 g/day/sow, Beijing Centre Biology Co., Ltd., Beijing, China). All sows were fed two times per day at 09:00 a.m. and 6:00 p.m. and all diets were consumed completely by all sows. After delivery, APS was withdrawn and all sows were fed the same diet. The dose of APS feed was determined according to our pilot trial results (unpublished data). All animal procedures were approved by the Ethical Committee of Hunan Agricultural University.

2.2. Sample Collection

Colostrum (3-4 mL per sample) was collected from the first teat of each sow at onset of parturition as 0 h-colostrum. Samples were also collected from the first teat at different time points postpartum (as 12 h- and 24 h-colostrum and 36 h-milk) by using individual artificial milking equipment. Sample collection from the fixed first teat was to avoid the value deviation caused by the location of teat as referred by others [17]. All samples were immediately frozen at −20°C before processing.

2.3. Quantitation of IgG and IgM

All samples were centrifuged at 5,000 gravity () for 30 min at 4°C, and supernatants were collected. IgG and IgM were measured using porcine IgG and IgM ELISA Quantitation Kits (Elabscience Biotechnology Co., Ltd., Wuhan, China) according to the manufacturer’s instruction.

2.4. Determination of CSFV Antibody

Classical Swine Fever Virus antibodies (CSFV Ab) were determined using a commercial ELISA Quantitation Kit (IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine, USA). The assay is a blocking ELISA using a microplate coated with CSFV antigen. Each sample was analyzed in duplicate, and the mean OD value of each tested sample () and that of the negative control () were calculated. The inhibition percentage of each sample was calculated according to the following formula: Blocking% = . The test sample was determined as positive with a blocking rate ≥40% (antibodies are present), or negative with a blocking rate ≤30% (antibodies are absent). Blocking rate was calculated according to the instructions as described by others [18].

2.5. Growth Factors EGF and IGF-1 Assay

Growth factors EGF and IGF-1 were assayed in colostrum and milk. Supernatants were diluted in 10-fold (for EGF assay) and 40-fold (for IGF-1 assay), respectively. The different dilutions of these growth factors were chosen according to our pilot trial results. The concentrations of the growth factors were quantified using swine EGF or IGF-1 ELISA Quantitation Kits (Elabscience Biotechnology Co., Ltd., Wuhan, China) according to the manufacturer’s instructions.

2.6. Statistical Analyses

SAS version 8 was used for the statistical analysis and the test of significance was calculated using two-way ANOVA analysis with Duncan’s multiple-range test. Results were presented as mean ± standard deviation (mean value ± SD). Difference was considered significant at a level of .

3. Results

As shown in Figure 1, prior to one week of the expected parturition, dietary supplementation of APS significantly enhanced the presence of IgG (Figure 1(a)) and IgM (Figure 1(b)) in 0 h-colostrum () at the onset farrowing and decreased with the time development after delivery. Concentrations of IgG and IgM in 12 h-colostrum were kept higher in APS group when compared to the control group (). Twenty-four hours postpartum, both IgG and IgM in colostrum showed no obvious difference between the APS and control groups.

(a)

(b)

In the present study, blocking rates against CSFV Ab were also determined. As shown in Table 2, CSFV antibody blocking rates from all test colostrum were above 30% with antibodies presence in 0 h- and 12 h-colostrum (blocking rates above 40%), which indicated that vaccination with a swine fever vaccine could obtain good immunity. In APS treated or control group, the blocking rates of CSFV Ab were decreased with the time development. With the supplementation of APS, blocking rates were increased in all indicated time points’ colostrum and milk when compared to the controls, and significant differences were observed as in 0 h-colostrum and as in other two time points’ colostrum and milk postpartum, respectively.

Growth factor concentrations of EGF and IGF-1 were measured as shown in Figure 2. Concentrations of EGF were rapidly decreased in colostrum postpartum when compared to the 0 h-colostrum (), and dietary supplementation with APS increased the EGF production in 0 h-colostrum () (Figure 2(a)). After farrowing, no difference in EGF was observed between colostrum collected on the indicated time points (Figure 2(a)). The growth factor IGF-1 showed a similar change as EGF that APS increased its production in 0 h-colostrum (), but it continued to decrease with the time development postpartum (Figure 2(b)).

(a)

(b)

4. Discussion

In the current field study, supplementation of APS in sow diet markedly improved the levels of IgM and IgG in colostrum one week before the expected delivery date. APS is used to be a immunomodulatory agent to improve growth and immune function in chickens and weaned pigs. Additives of APS in drinking water or diet could increase the average daily gain of chickens and weaned pigs [16, 19]. APS enhanced the production of serum IgM and IgG in aged mice and synergistically resisted the immunosuppression with epimedium polysaccharide in chickens, which are the major immunoglobulins products of humoral immune responses [20, 21]. Therefore, the increase of IgG and IgM upon the APS supplementation in the present study might result from the improvement of humoral immune in the current study.

Vaccination with appropriate vaccines is the main way to prevent the viral infectious diseases. Combined application of a vaccine with an adjuvant or immunomodulator could improve the efficacy of a vaccine. Classical swine fever (CSF) is a contagious viral disease, and mandatory vaccination against CSF is carried out in several countries worldwide. Studies showed that the presence of maternally derived antibodies can protect piglets against this disease [22, 23]. An in vitro study indicated that APS had immunomodulatory effects on peripheral blood mononuclear cells (PBMCs) exposed to CSFV [24]. In the present study, daily supplementation of APS before farrowing could increase the maternally derived specific antibody against CSFV in colostrum which suggested that APS might serve as an effective agent of vaccines against CSFV. Studies showed that APS has beneficial effects on immune response, improving the function of T-lymphocytes and increasing the proliferation of lymphocyte [24–26]. In the present study, the enhancement of CSFV antibody with the dietary APS supplementation in sow could be a result of the increase of the cellular immunity in animals.

Usually, blood is collected from the jugular vein to determine the status of some specific vaccination in sow, which can cause stress and experienced staff is needed in the process of sample collection. Due to relatively high concentrations of immunoglobulins in colostrum, the correlation of maternal E. rhusiopathiae- and M. hyopneumoniae-specific antibody ELISA levels was investigated; the results showed that colostrum has the potential to improve the sensitivity of both ELISAs when compared with serum [27]. In the present study, high levels of specific CSFV antibody were also measured in colostrum, which indicates that colostrum could be used a resource to determine the status of sow immunity.

In colostrum, high content of bioactive compounds such as growth factors, antioxidants, and antimicrobial and immune-modulatory factors can support intestinal maturation, balance and priming of the immune system and establishment of a beneficial gut microbiota [28, 29]. Bovine colostrum can restore intestinal function after initial formula-induced inflammation in preterm pigs [29]. EGF is one of the most abundant growth factors found in the milk and plays important roles in neonatal intestinal development to protect neonates against pathogens infection [30–32]. Plasma anabolic hormones, such as insulin and IGF-1, are highly associated with the local bovine mammary metabolism; highest levels of IGF-1 in milk were found 2 weeks antepartum followed by a rapid decrease during the first milking postpartum [33]. Studies indicated that neonatal tissue development may be improved when piglets consume colostrum with high growth factors such as IGF-1 [34, 35]. In the present study, growth factors EGF and IGF-1 were increased in sow colostrum upon supplementation of APS in diets which may promote the neonatal intestinal development.

In conclusion, APS can improve the concentrations of IgG, IgM, EGF, and IGF-1 in sow colostrum as well the levels of specific antibody against CFSV. Further studies need to be done to investigate the effects of APS on the development and maturation of the newborn piglet intestine.

Competing Interests

The authors declared no potential conflict of interests with respect to the research, authorship, and/or publication of this article.

Acknowledgments

This study was supported by grants from the Natural Science Foundation of China (31272630, 31572591, and 31201968) and the Hunan Natural Science Fund for Distinguished Young Scholars Project (2015JJ1009). The authors also thank Tom Yu for his assistance in manuscript preparation.

References

J. Le Dividich, J. A. Rooke, and P. Herpin, “Nutritional and immunological importance of colostrum for the new-born pig,” Journal of Agricultural Science, vol. 143, no. 6, pp. 469–485, 2005.
View at: Publisher Site | Google Scholar
J. A. Rooke and I. M. Bland, “The acquisition of passive immunity in the new-born piglet,” Livestock Production Science, vol. 78, no. 1, pp. 13–23, 2002.
View at: Publisher Site | Google Scholar
N. Devillers, C. Farmer, A.-M. Mounier, J. Le Dividich, and A. Prunier, “Hormones, IgG and lactose changes around parturition in plasma, and colostrum or saliva of multiparous sows,” Reproduction Nutrition Development, vol. 44, no. 4, pp. 381–396, 2004.
View at: Publisher Site | Google Scholar
F. Klobasa, E. Werhahn, and J. E. Butler, “Composition of sow milk during lactation,” Journal of animal science, vol. 64, no. 5, pp. 1458–1466, 1987.
View at: Publisher Site | Google Scholar
R. J. Xu, F. Wang, and S. H. Zhang, “Postnatal adaptation of the gastrointestinal tract in neonatal pigs: a possible role of milk-borne growth factors,” Livestock Production Science, vol. 66, no. 2, pp. 95–107, 2000.
View at: Publisher Site | Google Scholar
B. R. Westrom, B. G. Ohlsson, J. Svendsen, C. Tagesson, and B. W. Karlsson, “Intestinal transmission of macromolecules (BSA and FITC-Dextran) in the neonatal pig: enhancing effect of colostrum, proteins and proteinase inhibitors,” Biology of the Neonate, vol. 47, no. 6, pp. 359–366, 1985.
View at: Publisher Site | Google Scholar
R. Decaluwé, D. Maes, B. Wuyts, A. Cools, S. Piepers, and G. P. J. Janssens, “Piglets' colostrum intake associates with daily weight gain and survival until weaning,” Livestock Science, vol. 162, no. 1, pp. 185–192, 2014.
View at: Publisher Site | Google Scholar
H. Quesnel, C. Farmer, and N. Devillers, “Colostrum intake: influence on piglet performance and factors of variation,” Livestock Science, vol. 146, no. 2-3, pp. 105–114, 2012.
View at: Publisher Site | Google Scholar
S. Kallon, X. Li, J. Ji et al., “Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo,” Journal of Animal Science and Biotechnology, vol. 4, no. 1, article no. 22, 2013.
View at: Publisher Site | Google Scholar
J. Li, Y. Zhong, H. Li et al., “Enhancement of Astragalus polysaccharide on the immune responses in pigs inoculated with foot-and-mouth disease virus vaccine,” International Journal of Biological Macromolecules, vol. 49, no. 3, pp. 362–368, 2011.
View at: Publisher Site | Google Scholar
Q. Liu, Y. Yao, S. Zhang, and Z. Sheng, “Astragalus polysaccharides regulate T cell-mediated immunity via CD11c^highCD45RB^low DCs in vitro,” Journal of Ethnopharmacology, vol. 136, no. 3, pp. 457–464, 2011.
View at: Publisher Site | Google Scholar
P. Shao, L.-H. Zhao, Zhi-Chen, and J.-P. Pan, “Regulation on maturation and function of dendritic cells by Astragalus mongholicus polysaccharides,” International Immunopharmacology, vol. 6, no. 7, pp. 1161–1166, 2006.
View at: Publisher Site | Google Scholar
N. Zhang, J. Li, Y. Hu et al., “Effects of astragalus polysaccharide on the immune response to foot-and-mouth disease vaccine in mice,” Carbohydrate Polymers, vol. 82, no. 3, pp. 680–686, 2010.
View at: Publisher Site | Google Scholar
Y. Chen, D. Wang, Y. Hu et al., “Astragalus polysaccharide and oxymatrine can synergistically improve the immune efficacy of Newcastle disease vaccine in chicken,” International Journal of Biological Macromolecules, vol. 46, no. 4, pp. 425–428, 2010.
View at: Publisher Site | Google Scholar
L. A. Jaeger, C. H. Lamar, G. D. Bottoms, and T. R. Cline, “Growth-stimulating substances in porcine milk,” American Journal of Veterinary Research, vol. 48, no. 10, pp. 1531–1533, 1987.
View at: Google Scholar
S. L. Yuan, X. S. Piao, D. F. Li, S. W. Kim, H. S. Lee, and P. F. Quo, “Effects of dietary Astragalus polysaccharide on growth performance and immune function in weaned pigs,” Animal Science, vol. 82, no. 4, pp. 501–507, 2006.
View at: Publisher Site | Google Scholar
S. Ogawa, T. Tsukahara, T. Tsuruta et al., “The evaluation of secretion volume and immunoglobulin A and G concentrations in sow colostrum from anterior to posterior teats,” Animal Science Journal, vol. 85, no. 6, pp. 678–682, 2014.
View at: Publisher Site | Google Scholar
Z. Wang, Y. Shao, Y. Guo, and J. Yuan, “Enhancement of peripheral blood CD8+ T cells and classical swine fever antibodies by dietary β-1,3/1,6-glucan supplementation in weaned piglets,” Transboundary and Emerging Diseases, vol. 55, no. 9-10, pp. 369–376, 2008.
View at: Publisher Site | Google Scholar
N. Li, R. P. She, L. J. Han, and K. Z. Wang, “Effect of Astragalus root extractions on chicken growth and immunological function,” Chinese Journal of Veterinary Science & Technology, vol. 34, no. 5, pp. 61–64, 2004.
View at: Google Scholar
K. Kajimura, Y. Takagi, K. Miyano et al., “Polysaccharide of Astragali radix enhances IgM antibody production in aged mice,” Biological & Pharmaceutical Bulletin, vol. 20, no. 11, pp. 1178–1182, 1997.
View at: Publisher Site | Google Scholar
L. Guo, J. Liu, Y. Hu et al., “Astragalus polysaccharide and sulfated epimedium polysaccharide synergistically resist the immunosuppression,” Carbohydrate Polymers, vol. 90, no. 2, pp. 1055–1060, 2012.
View at: Publisher Site | Google Scholar
P. L. Eblé, S. Quak, Y. Geurts, H. W. M. Moonen-Leusen, and W. L. A. Loeffen, “Efficacy of CSF vaccine CP7_E2alf in piglets with maternally derived antibodies,” Veterinary Microbiology, vol. 174, no. 1-2, pp. 27–38, 2014.
View at: Publisher Site | Google Scholar
S. Suradhat, S. Damrongwatanapokin, and R. Thanawongnuwech, “Factors critical for successful vaccination against classical swine fever in endemic areas,” Veterinary Microbiology, vol. 119, no. 1, pp. 1–9, 2007.
View at: Publisher Site | Google Scholar
Z.-Y. Zhuge, Y.-H. Zhu, P.-Q. Liu et al., “Effects of astragalus polysaccharide on immune responses of porcine PBMC stimulated with PRRSV or CSFV,” PLOS ONE, vol. 7, no. 1, Article ID e29320, 2012.
View at: Publisher Site | Google Scholar
X. Kong, Y. Hu, R. Rui, D. Wang, and X. Li, “Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken,” International Immunopharmacology, vol. 4, no. 7, pp. 975–982, 2004.
View at: Publisher Site | Google Scholar
B.-M. Shao, W. Xu, H. Dai, P. Tu, Z. Li, and X.-M. Gao, “A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb,” Biochemical and Biophysical Research Communications, vol. 320, no. 4, pp. 1103–1111, 2004.
View at: Publisher Site | Google Scholar
C. J. Jenvey, M. P. Reichel, and P. D. Cockcroft, “Erysipelothrix rhusiopathiae and Mycoplasma hyopneumoniae: the sensitivities of enzyme-linked immunosorbent assays for detecting vaccinated sows of unknown disease status using serum and colostrum, and the correlation of the results for sow serum, colostrum, and piglet serum,” Journal of Veterinary Diagnostic Investigation, vol. 27, no. 2, pp. 211–216, 2015.
View at: Publisher Site | Google Scholar
R. J. Playford, C. E. Macdonald, and W. S. Johnson, “Colostrum and milk-derived peptide growth factors for the treatment of gastrointestinal disorders,” American Journal of Clinical Nutrition, vol. 72, no. 1, pp. 5–14, 2000.
View at: Google Scholar
A. C. F. Støy, P. M. H. Heegaard, T. Thymann et al., “Bovine colostrum improves intestinal function following formula-induced gut inflammation in preterm pigs,” Clinical Nutrition, vol. 33, no. 2, pp. 322–329, 2014.
View at: Publisher Site | Google Scholar
A. Bedford, T. Chen, E. Huynh et al., “Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: potential mechanisms involved,” Journal of Biotechnology, vol. 196-197, pp. 9–19, 2015.
View at: Publisher Site | Google Scholar
K. Jung, B.-K. Kang, J.-Y. Kim, K.-S. Shin, C.-S. Lee, and D.-S. Song, “Effects of epidermal growth factor on atrophic enteritis in piglets induced by experimental porcine epidemic diarrhoea virus,” Veterinary Journal, vol. 177, no. 2, pp. 231–235, 2008.
View at: Publisher Site | Google Scholar
R. T. Zijlstra, J. Odle, W. F. Hall, B. W. Petschow, H. B. Gelberg, and R. E. Litov, “Effect of orally administered epidermal growth factor on intestinal recovery of neonatal pigs infected with rotavirus,” Journal of Pediatric Gastroenterology & Nutrition, vol. 19, no. 4, pp. 382–390, 1994.
View at: Publisher Site | Google Scholar
D. Schams and R. Einspanier, “Growth hormone, IGF-I and insulin in mammary gland secretion before and after parturition and possibility of their transfer into the calf,” Endocrine Regulations, vol. 25, no. 1-2, pp. 139–143, 1991.
View at: Google Scholar
D. G. Burrin, T. J. Wester, T. A. Davis, S. Amick, and J. P. Heath, “Orally administered IGF-I increases intestinal mucosal growth in formula-fed neonatal pigs,” American Journal of Physiology, vol. 270, no. 5, pp. 1085–1091, 1996.
View at: Google Scholar
V. M. Houle, E. A. Schroeder, J. Odle, and S. M. Donovan, “Small intestinal disaccharidase activity and ileal villus height are increased in piglets consuming formula containing recombinant human insulin- like growth factor-I,” Pediatric Research, vol. 42, no. 1, pp. 78–86, 1997.
View at: Publisher Site | Google Scholar

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Copyright © 2017 Lunbo Tan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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