Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus
Table 5
Proposed epitope vaccine ensemble for EBV.
CD8 T cell epitope vaccine component
Sequence
Antigen
AN1
BLAST hit HMP2
BLAST hit humans3
PPC%4
RPPIFIRRL
EBNA3
P12977
EFI49553.1 (55.56)
NP_001182344.1 (66.67)
SVRDRLARL
EBNA3
P12977
No hit (—)
3HR0 (55.56)
YVLDHLIVV
BRLF1
P03209
EPH07203.1 (88.89)
XP_011535331.1 (66.67)
QPRAPIRPI
EBNA6
P03204
EEZ70880.1 (66.67)
AFC01212.1 (55.56)
LPCVLWPVL
BZLF1
P03206
ETN46892.1 (77.78)
XP_011511695.1 (55.56)
RVRAYTYSK
BRLF1
P03209
EEY91922.1 (66.67)
CAE46202.1 (55.56)
>95
AYSSWMYSY
EBNA3
P12977
EKB85112.1 (77.78)
EAW88404.1 (66.67)
VLKDAIKDL
EBNA1
P03211
KXB56071.1 (88.89)
EAX00446.1 (66.67)
QAKWRLQTL
EBNA3
P12977
EHR35488.1 (66.67)
XP_005255827.1 (77.78)
RRIYDLIEL
EBNA6
P03204
EDS12420.1 (77.78)
EAW88480.1 (66.67)
RLRAEAQVK
EBNA3
P12977
No hit (—)
XP_011507142.1 (77.78)
CYDHAQTHL
BMRF1
P03191
EFF75621.1 (77.78)
CAH10644.1 (66.67)
SENDRLRLL
BZLF1
P03206
EGG37664.1 (77.78)
EAW88969.1 (77.78)
YRSGIIAVV
BMRF1
P03191
OFQ99895.1 (88.89)
BAC03504.1 (66.67)
CD4T cell epitope vaccine component
Sequence
Antigen
AN1
BLAST hit HMP2
BLAST hit humans3
PPC%4
MLGQDDFIKFKSPLV
BFRF1
P03185
EIY33207.1 (46.67)
NP_001284364.1 (53.33)
AGLTLSLLVICSYLFISRG
BHRF1
P03182
EKN19533.1 (47.37)
EAW92092.1 (52.63)
>81.8
SRDELLHTRAASLLY
BARF1
P0CAP6
EPB87510.1 (66.67)
XP_011514101.1 (66.67)
PPVVRMFMRERQLPQ
EBNA6
P03204
EFV04068.1 (46.67)
AAP34452.1 (60.00)
B cell epitope vaccine component
Sequence
Antigen
AN1
BLAST hit HMP2
BLAST hit humans3
Src.5
APESTTTSPTLNTTGFA
gp350 (BLLF1)
P03200
EGY79509.1 (58.82)
NP_001276932.1 (52.94)
E
GNGPKASGGD
gp350 (BLLF1)
P03200
EHM51909.1 (70.00)
NP_055501.2 (70.00)
E
ETVPYIKWDN
gp350 (BLLF1)
P03200
EET62946.1 (50.00)
NP_001193968.1 (50.00)
E
KLPHWTPTLH
gp42 (BZLF2)
P03205
EJZ65106.1 (70.00)
AAH22472.1 (60.00)
P
NTTVGIELPDA
gpB (BALF4)
P03188
EHM53795.1 (72.73)
XP_011519547.1 (63.64)
P
SSHGDLFRFSSDIQCP
gpB (BALF4)
P03188
KGF26221.1 (50.00)
XP_011520599.1 (50.00)
P
FSVEDLFGAN
gL (BKRF2)
P03212
EKB09257.1 (80.00)
XP_005271219.1 (70.00)
P
1Accession number from UniProtKB database. 2,3Accession number of the closest epitope BLAST hit to human microbiome proteins and human proteins, respectively (percentage of identity in parenthesis). 4Population protection coverage (PPC) of the CD8 and CD4 T cell epitope ensemble. 5Src., source, whether the epitope derived from an experimental B cell epitope (E) or it was predicted (P).
