Propyl Gallate Exerts an Antimigration Effect on Temozolomide-Treated Malignant Glioma Cells through Inhibition of ROS and the NF-κB Pathway

Figure 3

Analysis of MMP-2 and MMP-9 activities with TMZ, PG, or TMZ/PG treatment. U87MG cells (2 × 10⁶) were plated in 100 mm cultured dishes for 24 h and then treated with TMZ (200 μM), PG (50 or 100 μM), or their combination for 48 h. After treatment, the cultured media were collected and then centrifuged at 12,000 ×g for 10 min at 4°C to obtain supernatants. Aliquot protein (50 μg) was used to evaluate the activities of MMP-2 and MMP-9 by zymography. These experiments were performed at least three times; a representative experiment is presented. Data indicate the densitometric values of various treated groups normalized to their corresponding untreated group. Significant differences for the untreated group and TMZ group were , , and ^#.