Propyl Gallate Exerts an Antimigration Effect on Temozolomide-Treated Malignant Glioma Cells through Inhibition of ROS and the NF-κB Pathway

Figure 4

Evaluation of intracellular ROS with TMZ, PG, or TMZ/PG treatment. U87MG cells (1 × 10⁶) were plated in 60 mm cultured dishes for 24 h and then treated with TMZ (200 μM), PG (50 or 100 μM), or their combination for 48 h. After treatment, the cells were stained with 2,7-dichlorfluorescein-diacetate (DCFDA) for ROS analysis and were then evaluated by flow cytometry. Data represent the mean fluorescence intensity within the cells. The values are presented as mean ± standard deviation (–8) of individual experiments. Significant differences for the untreated group and TMZ group were , , , ^#, and ^##.