Propyl Gallate Exerts an Antimigration Effect on Temozolomide-Treated Malignant Glioma Cells through Inhibition of ROS and the NF-κB Pathway

<div>Expressions of (a) p-IKK and IKK; (b) p-IκB, IκB, and p-p65; (c) nuclear p65 with TMZ, PG, or TMZ/PG treatment. U-87 MG cells (2 × 106) were plated in 100 mm cultured dishes for 24 h and then treated with TMZ (200 μM), PG (50 or 100 μM), or their combination for 48 h. After treatment, total proteins or nuclear proteins were extracted to assess various protein expressions. Fifty micrograms of protein were loaded onto a 12% SDS-polyacrylamide gel and evaluated by Western blotting. These experiments were performed at least three times; a representative experiment is presented. Data indicate the densitometric values of various treated groups normalized to their corresponding untreated group.</div>

Journal of Immunology Research

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Figure 5

Figure 5: Propyl Gallate Exerts an Antimigration Effect on Temozolomide-Treated Malignant Glioma Cells through Inhibition of ROS and the NF-κB Pathway

Figure 5 | Propyl Gallate Exerts an Antimigration Effect on Temozolomide-Treated Malignant Glioma Cells through Inhibition of ROS and the NF-κB Pathway 