Toll-Like Receptor Ligand-Induced Liver Injury in D-Galactosamine-Sensitized Mice: Differences between TLR7/8 and TLR9 Ligands, Cytokine Patterns, and Cross-Tolerance Induction by TLR2 Ligand Pretreatment
Table 3
Serum levels of cytokines (pg/ml) in a set of experiments with C57BL/6 mice. Mean ± SD is shown.
Cytokine
Time
PBS
CpG/D-galN†
R848/D-galN†
()
LTA− ()
LTA+ ()
LTA− ()
LTA+ ()
ALT (U/l)
68 ± 13 (10 h)
17875.7 ± 5156.2 (7.5–10 h)
202.4 ± 108.2 (10 h)
14737.5 ± 3894.0 (6–8.5 h)
10112.1 ± 5229.2 (8-10 h)
TNF-α
5 h
1.5 ± 0.3
22.7 ± 21.0
7.3 ± 0.5
15.5 ± 10.8
9.6 ± 3.4
10 h
n.t.
39.8 ± 13.0
3.0 ± 0.0
Not available (death)
n.t.
IL-6
1 h
2.4 ± 1.6
1984.7 ± 771.4
67.7 ± 29.7
1029.4 ± 927.3
668.6 ± 481.5
5 h
2.1 ± 0.8
1270.5 ± 1199.0
112.5 ± 51.4
297.7 ± 210.9
359.4 ± 294.2
10 h
n.t.
4424.81 ± 3718.7
33.2 ± 24.8
Not available (death)
n.t.
INF-γ
1 h
1.4 ± 0.6
1.3 ± 0.5
1.6 ± 1.2
1.1 ± 0.6
1.5 ± 0.5
5 h
1.3 ± 0.7
89.5 ± 74.6
13.7 ± 5.4
52.8 ± 33.1
54.0 ± 9.4
10 h
n.t.
128.51 ± 147.2
6.5 ± 4.8
Not available (death)
n.t.
IL-10
1 h
n.t.
23.7 ± 18.6
6.2 ± 8.3
6.9 ± 4.3
2.7 ± 1.7
5 h
n.t.
90.2 ± 93.1
63.2 ± 62.0
26.6 ± 10.8
6.4 ± 4.7
10 h
n.t.
270.01 ± 249.5
2.0 ± 0.0
Not available (death)
n.t.
IL-17A
1 h
n.t.
2.1 ± 1.1
2.4 ± 2.2
1.75 ± 1.0
2.0 ± 1.3
5 h
n.t.
2.6 ± 2.1
4.9 ± 3.5
1.1 ± 0.8
2.7 ± 0.8
10 h
n.t.
1.01 ± 0.6
1.0 ± 0.0
Not available (death)
n.t.
IL-23
1 h
n.t.
16.7 ± 3.4
10.5 ± 3.7
12.4 ± 4.7
14.3 ± 7.4
5 h
n.t.
15.0 ± 4.6
16.5 ± 3.7
9.4 ± 3.8
16.7 ± 3.4
10 h
n.t.
18.31 ± 8.3
9.0 ± 0.0
Not available (death)
n.t.
IL-27
1 h
n.t.
4.6 ± 2.5
7.5 ± 5.0
2.6 ± 1.4
4.7 ± 2.6
5 h
n.t.
8.2 ± 4.6
11.0 ± 8.2
3.1 ± 1.5
10.3 ± 5.6
10 h
n.t.
5.51 ± 3.0
2.4 ± 1.1
Not available (death)
n.t.
n.t.: not tested. †PBS (LTA− mice) or 200 μg/mouse LTA (LTA+ mice) was administered 24 h prior to the challenge with the 20 μg CpG (or 10 μg R848)/20 μg D-galN. Asterisks indicate a significant difference between with and without the LTA pretreatment (,, and ). 1Two out of seven mice died within 10 h, and the five mice that survived within 10 h were analyzed.
