Fn14 Deficiency Ameliorates Anti-dsDNA IgG-Induced Glomerular Damage in SCID Mice

Figure 6

Proinflammatory cytokines and pEGFR are downregulated in Fn14-deficient kidneys. Both WT and KO mice were injected with anti-dsDNA IgG hybridoma cells. (a) By real-time PCR, the mRNA levels of regulated on activation, normal T cell expressed and secreted (RANTES), monocyte chemotactic protein 1 (MCP-1), interferon gamma-induced protein 10 (IP-10), and epidermal growth factor receptor (EGFR) were determined in kidneys. (b) By Western blotting, the proteins of RANTES, MCP-1, IP-10, and phospho-EGFR (pEGFR) were determined in the kidney lysates. (c) The intensities of blots were measured by ImageJ software. (d) Urine MCP-1 levels were determined in different groups. (e) Glomerular pEGFR expression was also detected by immunohistochemistry. (f) The pEGFR-stained sections were scored semiquantitatively. There were eight mice in each group. Representative images are shown. Fn14: fibroblast growth factor-inducible 14. Scale . and .

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