Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A

Figure 2

miR-362 promotes NSCLC metastasis in vitro and in vivo. (a, b) Cell invasion and migration ability decreased when miR-362 was absent in A549. (c, d) Cell invasion and migration ability decreased when miR-362 was absent in 95-D. (e, g) Transwell invasion and migration assays were employed in A549 cell lines transfected by miR-362. (f, h) Transwell invasion and migration assays were employed in 95-D cell lines transfected by miR-362. miR-362 concentrations were 20 nm and 50 nm. Cells were stained and counted using light microscopy (magnification, 200x). (i) Inhibition of miR-362 suppressed tumor formation in vivo. Representative images of xenograft tumors. (j) Tumor volumes were measured every two days. (a–h) Data were from three independent experiments. , , and .

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