Deoxycholic Acid-Mediated Sphingosine-1-Phosphate Receptor 2 Signaling Exacerbates DSS-Induced Colitis through Promoting Cathepsin B Release

Figure 3

Blockage of ERK signaling prevents ASC speck formation and mature IL-1β secretion. (a) LPS-primed J774A.1 macrophages were treated with DCA in the presence or absence of JTE-013 or U0126. The cells were then stained with ASC antibody and DAPI. Representative immunofluorescent images of ASC specks were shown. (b) LPS-primed J774A.1 macrophages were treated with DCA in the presence or absence of JTE-013 or U0126 for 24 h. Immunoblot was performed to determine mature IL-1β (mIL-1β, 17kD) in the culture supernatants (SN) and precursors of IL-1β (pro-IL-1β) in cell lysates, and (c) secreted IL-1β in supernatants was also analyzed by ELISA. and . Error bars indicate s.e.m. The data shown are representative of 3 independent experiments.

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