OSU-T315 as an Interesting Lead Molecule for Novel B Cell-Specific Therapeutics

Table 2

In vitro phenotypic characterization of OSU-T315 on splenic B cells from wild type mice and mice with B cell-specific deletion of ILK.

Stimulus	Readout	Wild type B cells		ILK KO B cells
		IC50 (nM)	SEM (nM)	IC50 (nM)	SEM (nM)
ODN1826	IgM	215	21	220	10
	IgG	200	75	135	40
Resiquimod	IgM	235	30	200	20
	IgG	215	65	125	15
LPS	IgM	230	65	210	90
	IgG	235	65	180	100
	IL6	80	20	60	15
	TNFα	470	130	690	150
	Proliferation	225	55	105	35

Murine B cells were stimulated with 0.3 μM ODN1826, 1 μM resiquimod, or 5 μg/ml LPS in the presence of different concentrations of OSU-T315. After 7 days of incubation, supernatant was taken for analysis of IgM and IgG. LPS-induced production of IL6 and TNFα was measured after 2 days of incubation. The proliferation rate of LPS-activated murine B cells was measured by ³H thymidine incorporation after 3 days of incubation. Data are the means with SEM of 2 independently performed experiments.