HLA-A2-Restricted Epitopes Identified from MTA1 Could Elicit Antigen-Specific Cytotoxic T Lymphocyte Response

Figure 5

Specific lysis of various cell lines and IFN-γ secretion by induced T cells in HLA-A2.1/K^b transgenic mice immunized with MTA1_(1–283). MTA1_(1–283) (100 μg/ml) or the same volume of PBS (negative control) emulsified in IFA was used to immunize HLA-A2.1/K^b transgenic mice on days 0 and 7. On day 14, the mice were sacrificed, and their splenocytes were collected and restimulated for another 6 days with the peptide pool (10 μg/ml) and 50 U/ml rmIL-2 in vitro. (a) Serum was collected from the immunized mice on day 14, and the serum IFN-γ concentration was measured by ELISA. (b) The percentage of IFN-γ secreting peptide-specific CD8⁺ T cells induced by MTA1_(1–283) and PBS was analyzed by an intracellular staining assay. (c) The percentages of IFN-γ⁺CD4⁺ T cells in the MTA1_(1–283) and PBS groups were determined. (d) Specific lysis of target cells SW620 cells (HLA-A2⁺, MTA1⁺) was assessed by the LDH cytotoxicity assay. and . vs. the control group. Data represent means ± SD ().

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