Venom from Bothrops lanceolatus, a Snake Species Native to Martinique, Potently Activates the Complement System

Figure 4

Cleavage of purified Complement regulator C1-INH by B. lanceolatus venom and its residual C1s-complexing activity. C1-INH (2 μg) samples were incubated with B. lanceolatus venom (2 μg) for 30 min at 37°C and the cleavage was visualized by SDS-PAGE electrophoresis, followed by silver staining (a), or by Western blot using anti-C1-INH antibodies (b). The residual C1s-complexing activity of the C1-INH fragments was assessed by functional ELISA (c). The results represent the mean of two independent experiments, carried out in triplicates. Data are represented as mean ± SD. between two samples.

(a)

(b)

(c)