Research Article

Mycobacterium tuberculosis Protein Rv3841 Activates Dendritic Cells and Contributes to a T Helper 1 Immune Response

Figure 1

Rv3841 induces DC maturation. (a) Rv3841 induced functional activation of DCs in a dose-dependent manner. Immature DCs (106 cells/mL) were cultured in the presence of 1, 5, or 10 μg/mL Rv3841 or 100 ng/mL LPS for 24 h. The quantities of TNF-α, IL-1β, IL-10, and IL-12p70 in the culture supernatant were determined by ELISAs. All the data were expressed as mean ± SD (). The levels of significance (, , or determined by one-way ANOVA) of the differences between the treatment data and the control data are indicated; treatments that were not significantly different are indicated by “n.s.” (b) Rv3841 induced phenotypic and functional activation of DCs in a dose-dependent manner, and the cells were analyzed for the expression of surface markers by flow cytometry. The cells were gated on CD11c+. The DCs were stained with an anti-CD80, anti-CD86, anti-MHC class I, or anti-MHC class II antibody. The percentage of positive cells is shown in each panel. The bar graphs depict data as mean ± SD (). The levels of significance ( or , determined by one-way ANOVA) of the differences between the treatment data and the control data are indicated. Treatments without a significant effect are indicated by “n.s.”
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