Mycobacterium tuberculosis Protein Rv3841 Activates Dendritic Cells and Contributes to a T Helper 1 Immune Response

Figure 6

Rv3841-treated DCs induce expansion of the effector/memory T-cell population. DCs from WT mice were treated with Rv3841 (10 μg/mL) or LPS (100 ng/mL) and then cocultured for 3 days with T-cells from Mtb-infected mice at the DC to T-cell ratio of 1 : 10. Splenocytes were stained with anti-CD4, anti-CD62L, and anti-CD44 monoclonal antibodies. (a, b) A histogram is shown for gating of the labeled T-cells. Bar graphs show CD62L^lowCD44^high T-cells or CD62L^highCD44^low T-cell populations among the spleen cells. (c) Culture supernatants were harvested after 96 h, and IFN-γ, IL-2, and IL-4 concentrations were measured by ELISAs. The mean values ± SD () are shown; , or : a significant difference of treatment groups from the appropriate controls, as determined by one-way ANOVA test. Treatments without a significant effect are indicated by “n.s.”

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