Downregulation of Interleukin- (IL-) 17 through Enhanced Indoleamine 2,3-Dioxygenase (IDO) Induction by Curcumin: A Potential Mechanism of Tolerance towards Helicobacter pylori

Figure 4

Levels of IL-17 by Western blotting in total protein extracts from gastric biopsies of the subgroup of H. pylori-infected patients () treated with medium (untreated samples) or 200 μM curcumin or 200 μM curcumin plus the indoleamine 2,3-dioxygenase (IDO) inhibitor, 1-methyl-L-tryptophane (1-MT), at a concentration of 200 μM for 20 hours. The loading control was represented by β-actin. Values are expressed as mean values ± SD of arbitrary units (a.u.). A Western blot panel of one representative experiment is shown (A is the untreated sample, B is the curcumin-treated sample, and C is the curcumin plus 1-MT-treated sample, for each patient). Statistical analysis was performed using one-way analysis of variance (ANOVA) with the Tukey multiple comparison test. values of 0.05 or less were regarded as statistically significant.