March1 E3 Ubiquitin Ligase Modulates Features of Allergic Asthma in an Ovalbumin-Induced Mouse Model of Lung Inflammation

Figure 1

Deficiency of MARCH1 in MARCH1^−/− mice upregulates MHC II surface expression in lung myeloid and lymphoid immune cells. (a) Gating strategy illustrating analysis of the isolated and stained lung immune cells by flow cytometry for MHC II MFI assessment. (b) A representative comparison of MHC class II expression on WT and March1^−/− CD11b-negative cells is shown. (c) MHC class II MFI of lung cells from WT and March1^−/− mice. (d) MHC class II MFI of lung cells from WT and March1^−/− mice in OVA-induced allergic model. For each population, MFIs were normalized to the value obtained for WT mice sensitized and challenged with OVA. , , , and (trend). Data are presented as means with SEM; /group in (c) (one experiment); data are pooled from 4 independent experiments in (d), total -9/group.

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