Stimulation of Dectin-1 and Dectin-2 during Parenteral Immunization, but Not Mincle, Induces Secretory IgA in Intestinal Mucosa

Figure 1

NF-κB-, AP-1-, and NFAT-dependent luciferase reporter gene expressions in RAW-NF-κB-Luc, RAW-NFAT-Luc, and RAW-AP-1-Luc macrophages, correspondingly, treated for 8 h with the indicated doses (μg/mL) of Mincle, Dectin-1, and Dectin-2 agonists. Data are mean ± SD fold increase over PBS-treated cells from three independent experiments, each performed in duplicates. Data were compared using the two-tailed unpaired Mann–Whitney t-test. # indicates significant differences in luciferase expression between control (PBS-treated) and TDB-, curdlan-, or furfurman-treated cells (^#; ^##). indicates significant differences in luciferase expression between Mincle-, Dectin-1-, and Dectin-2-stimulated cells (; ; ).