Stimulation of Dectin-1 and Dectin-2 during Parenteral Immunization, but Not Mincle, Induces Secretory IgA in Intestinal Mucosa
Mincle, Dectin-1, and Dectin-2 agonists induce comparable antigen-specific CD4+ and CD8+ T cell response. Mice were immunized twice by subcutaneous injection of ovalbumin alone or in combination with TDB, curdlan, or furfurman. Naïve mice were used as negative control. Splenocytes were harvested 14 days after the second immunization, labeled with CFSE, restimulated with 1 μg/mL ovalbumin for 72 h, stained with fluorescently tagged antibodies against CD3, CD4, and CD8, and analyzed by flow cytometry. (a) Gating strategy to quantify proliferating CD4+ and CD8+ T cells. (b) Minimum, maximum, and median of the abundance of ovalbumin-specific proliferating CD4+ and CD8+ T cells from two independent experiments with 5 mice per group per experiment. Data were compared using the two-tailed unpaired Mann–Whitney t-test. #;## relative to control group. ; between Mincle-, Dectin-1-, and Dectin-2-stimulated cells.