CD4^hiCD8^low Double-Positive T Cells Are Associated with Graft Rejection in a Nonhuman Primate Model of Islet Transplantation

Figure 3

DP T cells show helper functions and cytotoxic activity in vitro. After sorting T cells, CD4, CD8, and DP T populations were stimulated with PMA/ionomycin and stained with antibodies specific for (a) IFN-γ, (b) IL-4, and (c) IL-21 (; aged between 48 and 54 months). The percentage of T cells positive for (d) HLA-DR and (e) CXCR5/PD-1 is indicated (; aged between 48 and 54 months). Sorted cells were stimulated with PMA/ionomycin and then stained with antibodies specific for (f) granzyme B and (g) perforin (; aged between 48 and 54 months). Data from one independent experiment (left panels) and cumulative data (right panel) are shown. Data are expressed as the mean ± SD. and . n.s.: not significant.

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