Research Article

Proteomic Identification of Heat Shock-Induced Danger Signals in a Melanoma Cell Lysate Used in Dendritic Cell-Based Cancer Immunotherapy

Table 3

Currently known functions of gene-proteins exclusively identified in HS-conditioned samples (TRIMEL).

GeneID (NCBI)Full name (NCBI)Function (gene ontology)Reference

AHSG197Alpha 2-HS glycoprotein(i) Kinase inhibitor activity[89]
ANAPC164682Anaphase promoting complex subunit 1

ARID2196528AT-rich interaction domain 2(i) Protein binding[90]

ATAD3B83858ATPase family, AAA domain containing 3B

CLTB1212Clathrin light chain B(i) Protein binding[91]

CNIH10175Cornichon family AMPA receptor auxiliary protein 1

DOCK31795Dedicator of cytokinesis 3(i) Protein binding[92]

EEF1E19521Eukaryotic translation elongation factor 1 epsilon 1(i) Protein binding[93]

FLNC2318Filamin C(i) Ankyrin; cytoskeletal protein and protein binding[9496]

FUNDC265991FUN14 domain containing 2

GK22712Glycerol kinase 2(i) Glycerol kinase activity[97]

HEXIM110614Hexamethylene bisacetamide inducible 1(i) Cyclin-dependent protein serine/threonine kinase inhibitor activity[98101]
(ii) 7SK snRNA; protein and snRNA binding

HIST2H2AA3/HIST2H2AC8337/8338Histone cluster 2 H2A family member a3/Histone cluster 2 H2A family member c

HSD17B87923Hydroxysteroid 17-beta dehydrogenase 8(i) 3-Hydroxyacyl-CoA dehydrogenase; 3-oxoacyl-[acyl-carrier-protein] reductase (NADH) and estradiol 17-beta-dehydrogenase activity[102104]
(ii) NADH and protein binding

INPPL13636Inositol polyphosphate phosphatase like 1(i) SH2 domain and protein binding[105, 106]

ISOC279763Isochorismatase domain containing 2(i) Protein binding[107]

KIFC13833Kinesin family member C1(i) Microtubule motor activity[108]
(ii) ATP binding

LTN126046Listerin E3 ubiquitin protein ligase 1(i) Protein binding[109]

NSUN555695NOP2/Sun RNA methyltransferase family member 5(i) RNA binding[44, 45]

OAS149382-5′Oligoadenylate synthetase 1(i) 2-5-Oligoadenylate synthetase activity[93, 110112]
(ii) ATP; double-stranded RNA and protein binding

POLR1B84172RNA polymerase I subunit B(i) Protein binding[113]

PPP2R45524Protein phosphatase 2 phosphatase activator(i) Contributes to ATPase; protein heterodimerization; protein homodimerization; protein phosphatase regulator and protein tyrosine phosphatase activator activity[93, 114116]
(ii) ATP; protein, protein phosphatase 2A and receptor binding

RRP823378Ribosomal RNA processing 8, methyltransferase, homolog (yeast)(i) S-Adenosylmethionine-dependentmethyltransferase activity[44, 45, 117]
(ii) RNA; methylated histone and protein binding

SIGMAR110280Sigma nonopioid intracellular receptor 1(i) Drug binding[118]

SPATA5166378Spermatogenesis associated 5

SREK1140890Splicing regulatory glutamic acid and lysine rich protein 1(i) RNA and protein binding[45, 93]

SYNGR19145Synaptogyrin 1(i) Protein binding[93]

TMEM87A25963Transmembrane protein 87A

TRMT11251504tRNA methyltransferase 11-2 homolog (S. cerevisiae)(i) Protein methyltransferase activity[119121]
(ii) Protein binding

UAP16675UDP-N-acetylglucosamine pyrophosphorylase 1(i) Identical protein binding[93]

XPO623214Exportin 6(i) Protein transporter activity[122]
(ii) Protein binding

ID, identification number; NCBI, National Center for Biotechnology Information.