Anti-ICOSL New Antigen Receptor Domains Inhibit T Cell Proliferation and Reduce the Development of Inflammation in the Collagen-Induced Mouse Model of Rheumatoid Arthritis

Figure 2

Cell-based binding and ICOS/ICOSL blocking assays. Lead anti-mICOSL and anti-hICOSL VNAR-Fc proteins were tested for binding to the CHO cell surface expressed ICOSL and their corresponding efficacy in an ICOS/ICOSL blocking assay. (a) Titration curves of anti-mICOSL VNAR-Fc domains binding to mICOSL-CHO cells. (b) Titration curves of anti-hICOSL VNAR-Fc domains binding to hICOSL-CHO cells. (c) Concentration-dependent inhibition of mICOSL-Fc binding to cell surface expressing mICOS by the addition of serial dilutions of anti-mICOSL VNAR-Fc domains. (d) Concentration-dependent inhibition of hICOSL-Fc binding to cell surface expressing hICOS by the addition of serial dilutions of anti-hICOSL VNAR-Fc domains. 2V-Fc is the VNAR isotype control in each experiment.

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