A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies

Figure 3

Purification of the mAbs against rAra h 2. (a) Clone 1-2E10 purification graph. (b) Clone 2-1D5 purification graph. (c) Clone 3-1C5 purification graph. (d) Clone 4-1C2 purification graph. (e) Clone 5-1G4 purification graph. (The arrows indicate the eluted peak of the target protein.) (f) The mAbs after purification. M: prestained protein marker. Lane 1: 1-2E10. Lane 2: 2-1D5. Lane 3: 3-1C5. Lane 4: 4-1C2. Lane 5: 5-1G4.

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