DNA Methyltransferase Inhibition Promotes Th1 Polarization in Human CD4⁺CD25^high FOXP3⁺ Regulatory T Cells but Does Not Affect Their Suppressive Capacity

Figure 3

Effect of DAC on cytokine-producing potential of CD4⁺ Treg and Tconv. Flow cytometric analysis of αCD3/αCD28⁺ 100U rIL-2-stimulated FACS-sorted Treg and Tconv in the absence or presence of 1 μM of DAC, at day 8 of culture. The cytokine-producing potential was analyzed following 4 h stimulation with PMA/ionomycin in the presence of Brefeldin A. (a) Gating strategy. (b) Representative dot plots showing IFNγ-, IL-17a-, and IL-10-producing Treg and Tconv that were cultured in the presence or absence of DAC. (c) Cumulative data on IFNγ-, IL-17a-, and IL-10-producing Treg and Tconv after culture in the presence of absence of 1 μM DAC. (d) IFNγ production in FOXP3⁻ and FOXP3⁺ Treg, representative dot plots, and cumulative data are shown. are shown. , Wilcoxon signed-rank test, 2-tailed. .

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