Carbamazepine-Mediated Adverse Drug Reactions: CBZ-10,11-epoxide but Not Carbamazepine Induces the Alteration of Peptides Presented by HLA-B15:02

Figure 2

CBZ and EPX bind to the HLA heavy chain and not to the peptides. sB15:02 molecules were produced in the HLA class I-negative LCL721.221 cell line without treatment and in the presence of CBZ or EPX and purified via affinity chromatography. The amount of the drugs was measured mass spectrometrically (CBZ shown as ★, EPX shown as ◆). The threshold for drug detections is represented by a dashed line. The trimeric complexes (a) were analyzed as well as retentate and peptide fractions after separation of low (b) and high binding peptides (c). The amount of drug measured in the HLA heavy-chain fraction is displayed in black; the amount measured in the peptide fraction is shown in grey.

(a)

(b)

(c)