Research Article
miR-125a Suppresses TrxR1 Expression and Is Involved in H2O2-Induced Oxidative Stress in Endothelial Cells
Table 1
Primers for vector construction and quantitative PCR.
| | Name | Sequence |
| | pGL3-3UTR-forward | 5-CATTTGCAATGGAAAACACG-3 | | pGL3-3UTR-reverse | 5-TGCCTCAATTGCTCTCTCCT-3 | | Mutant-forward | 5-TACATTTCTGGGCCACCTCAGTCA ACCCATGCA T-3 | | Mutant-reverse | 5-CAGGCAGATGCATGGGTTGACTGAGGTGGCCCAG-3 | | pmiR-125a-forward | 5-TCCCTCTTATTCTGGCTTTC-3 | | pmiR-125a-reverse | 5-CATCCCAACAAACATCTGG-3 | | miR-125a-forward | 5-ACACTCCAGCTATATCCCTGAGACCCTTTA-3 | | miR-125a-reverse | 5-GGTGTCGTGGAGTCGGC-3 | | U6-forward | 5-CTCGCTTCGGCAGCACA-3 | | U6-reverse | 5-AACGCTTCACGAATTTGCGT-3 | | miR-125a RT primer | 5-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCACTGGT-3 |
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