Figure 2: Eutopic and ectopic endometrial homogenates induced immunological tolerance of THP-1 cells. THP-1 cell-derived macrophages were treated with either eutopic or ectopic endometrial homogenate or normal endometrial homogenate for 72 h and then stimulated by LPS for 24 h. According to flow cytometric analysis (a) and RT-PCR, the percentage of CD86+ cells (b) as well as CD86 mRNA (c) and IL-12 mRNA (d) levels was significantly decreased in THP-1 cell-derived macrophages treated with eutopic or ectopic endometrial homogenate from endometriosis patients, as compared with their counterparts. LPS: lipopolysaccharide. , , .