Mesenchymal Stem Cells Ameliorate Hepatic Ischemia/Reperfusion Injury via Inhibition of Neutrophil Recruitment

Figure 3

MSCs affect cytokine expression and reduce neutrophil infiltration. (a, e, f) The relative mRNA levels of IL-2, IL-4, IL-6, IL-10, CXCL2, CD11b, and CD18 genes in I/R lobes were detected by qRT-PCR. Data were normalized to glyceraldehyde-3-phosphate dehydrogenase gene expression. (b) The concentration of IL-2, IL-4, IL-6, IL-10, and CXCL2 in I/R lobes was measured by ELISA. (c) Representative images of immunohistochemistry-stained sections of MPO⁺ cells of liver tissues were shown. (d) The mean frequency of hepatic MPO⁺ cells in 10 high-power fields was calculated. Data are mean ± SD. , , . Original magnification, ×200.

(a)

(b)

(c)

(d)

(e)

(f)