Research Article
Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells
Figure 9
Effect of P2X7, FPR2, and EGFR inhibitors on LL-37-induced (a) TLR2 and (b) TLR4 expression. Mast cells were preincubated with P2X7 antagonist KN-62 at 1 μM, FPR2 antagonist PBP10 at 1 μM, or EGFR inhibitor AG1478 at 5 μM or medium alone for 15 min prior to stimulation with LL-37 at 1 μg/mL for 3 h. Representative images showing TLR cellular localization analyzed by confocal microscopy in nonpermeabilized cells. Single confocal sections (midsection of cells) reveal the presence of receptors. The signal was visualized with green Alexa 488. Fluorescence intensity diagrams showing the distribution of fluorescence in cells were mounted.
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