TLR3 Modulates the Response of NK Cells against Schistosoma japonicum

Figure 3

IFN-γ and IL-4 secretion induced by TLR agonists. (a) Splenic lymphocyte suspensions from normal and infected mice were prepared and cultured with PGN, Poly I:C, or LPS. Seventy-two hours later, IFN-γ or IL-4 production was assessed by using ELISA. (b) Splenic lymphocyte suspensions from normal and infected mice were prepared and stimulated with PGN, Poly I:C, LPS, or PMA plus ionomycin for 5 hours. IFN-γ and IL-4 production by splenic NK cells was assessed by using flow cytometry. Results of IFN-γ and IL-4 produced by splenic NK cells are calculated and shown (c). Data are shown as mean + SEM of 5 samples from three independent experiments. , comparison between the normal and infected groups.

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