Crotoxin Isolated from Crotalus durissus terrificus Venom Modulates the Functional Activity of Dendritic Cells via Formyl Peptide Receptors

Figure 3

CTX and CB induce an increase of PGE₂ and LXA₄ secretion by DCs incubated with LPS in vitro. DCs from BALB/c mice were stimulated with CTX, CA, or CB (250 ng/mL); LPS (250 ng/mL); and LPS + CTX, CB, and CA (250 ng/mL + 250 ng/mL). After 18 hours, the cell supernatants were collected for detection of PGE₂ (a) and LXA₄ (b) by ELISA. All samples were performed in quadruplicate and the results were expressed as the mean of the eicosanoid concentration ± SD. The dashed line represents the detection limit of each eicosanoid determined from the standard curve. The results are representative of three independent experiments. Statistical analyses were performed by one-way ANOVA, followed by Tukey’s test (GraphPad Prism 5.0, GraphPad Software). —LPS + CTX and LPS + CB groups compared with the LPS group.

(a)

(b)