Crotoxin Isolated from Crotalus durissus terrificus Venom Modulates the Functional Activity of Dendritic Cells via Formyl Peptide Receptors

Figure 6

FPRs are involved in the modulatory effect of CTX and CB on the DC maturation induced by LPS. DCs differentiated in vitro from bone marrow of mice were incubated or not with Boc-2 (100 μM) for 15 minutes. After this, the cells were centrifuged and added with the culture medium containing LPS (250 ng/mL), LPS + CTX, or LPS + CB (250 ng + 250 ng/mL) for 18 h. After this, the cells (10⁶) were labeled with anti-CD40 antibody (a), anti-CD80 (b), anti-CD86 (c), and anti-MHC II (d) and analyzed by flow cytometer. DC samples were also incubated with anti-CD11c-PE antibody or PE isotype control. The results were expressed as the mean fluorescence intensity (MIF) of DCs in quadruplicate ± SD. The data are representative of two independent experiments. Statistical analyses were performed by one-way ANOVA, followed by Tukey’s test (GraphPad Prism 5.0, GraphPad Software). —groups of DCs incubated with LPS compared with cells maintained in culture; —LPS + CTX and LPS + CB groups compared with the LPS group.

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