Fagonia indica Repairs Hepatic Damage through Expression Regulation of Toll-Like Receptors in a Liver Injury Model

Figure 4

Gene expression analysis of proinflammatory cytokines and hepatic markers with or without injury after treatment with Fagonia indica. (a) Proinflammatory cytokines: (A1) gel electrophoresis of conventional PCR products performed for IL-1β, IL-6, TNF-α, and TGF-β. qRT-PCR expression analysis to quantify the changes in mRNA expressions of (A2) IL-1β, (A3) IL-6, (A4) TNF-α, and (A5) TGF-β. (b) Hepatic injury markers: (B1) gel electrophoresis of conventional PCR products performed for col-1α and α-SMA. qRT-PCR expression analysis to quantify the changes in mRNA expressions of (B2) col-1α and (B3) α-SMA. (c) Normal hepatic markers: (C1) gel electrophoresis of conventional PCR products performed for albumin and krt-18. qRT-PCR expression analysis to quantify the changes in mRNA expressions of (C2) albumin and (C3) krt-18. Data was normalized with β-actin as housekeeping control. Graphs presented show fold change in gene expression of candidate genes as (, , and ). Statistical analysis was performed using one-way ANOVA with Tukey’s posttest.

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