Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection

Figure 6

Vaccination effects on the cardiac muscle sections. (a) Tissue sections were stained with hematoxylin and eosin or Masson’s trichrome and visualized by light microscopy (original magnification: 40x). Representative micrographs of the heart tissue from control-infected mice and mock-immunized with PBS (A), rTcENO (B), pBK-CMV empty plasmid vector (C), and pBK-TcENO (D) are shown. Black arrows show amastigote nests. Black arrowheads show inflammatory infiltrates. (b) Inflammatory lesion (lymphocyte infiltrates) scores. The inflammatory score was derived from two different experiments as described in Materials and Methods. The data are expressed as mean ± SD, and significance is presented as and (control group nonimmunized and infected versus vaccinated and infected groups, rTcENO and pBK-TcENO; rTcENO versus pBK-TcENO).

(a)

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