Research Article
Constitutive Changes in Circulating Follicular Helper T Cells and Their Subsets in Patients with Graves’ Disease
Figure 1
Flow analysis of circulating Tfh cells in GD patients. Human PBMCs from GD patients (BT: 36; AT: 21) and 20 HC were stained with anti-CD4, anti-CXCR5, anti-ICOS, anti-CD45RA, and anti-PD-1. (a) The cells were gated initially on lymphocytes and then circulating Tfh cells were analyzed by flow cytometry; (b) the numbers of circulating CD4+CXCR5+CD45RA−Tfh (cTfh) cells; (c) the numbers of CD4+CXCR5+CD45RA−ICOS+T cells; (d) CD4+CXCR5+CD45RA−PD-1+Tfh cells; (e) the correlation between PD-1+Tfh cell proportions and TPO-Ab levels in GD patients. , , and ; ns: no significant difference.
(a) |
(b) |
(c) |
(d) |
(e) |