Effect of CXCR2 Inhibition on Behavioral Outcomes and Pathology in Rat Model of Neuromyelitis Optica

Figure 1

(a, b) After immunizing Lewis rats with gpMBP to induce EAE, CXCR2 antagonist (red triangles) or vehicle control (green circles) was injected at the time points indicated by the black arrows and pooled IgG from AQP4-seropositive NMO patients was injected at the time points indicated by the pink arrows. Treatment with corticosteroid, Depo-Medrol, on days 8 and 10 was included in cohort 1 ((a) blue squares), while cohort 2 tested SCH527123 at both 10 mg/kg (orange squares) and 30 mg/kg (red triangles). EAE scores were not exacerbated by IgG from AQP4-seropositive NMO patients. (c) Diffusion of human IgG (Hu-IgG) into the spinal cord was assessed by immunohistochemistry (brown stain) and scored semiquantitatively. (d) Human IgG (Hu-IgG) was associated with higher EAE scores, while lower EAE scores were associated with lower levels of human IgG signal in the spinal cord.

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