Evaluation of an Automated Screening Assay, Compared to Indirect Immunofluorescence, an Extractable Nuclear Antigen Assay, and a Line Immunoassay in a Large Cohort of Asian Patients with Antinuclear Antibody-Associated Rheumatoid Diseases: A Multicenter Retrospective Study
Table 4
Multivariate analysis of the outcomes of antinuclear antibody-associated rheumatoid disease.
Dependent variables
Covariate
OR
SE
95% CI
value
Total AARD ()
Age (years)
0.9784
0.0081
0.9631 to 0.9940
0.0069
Female sex
0.3754
0.8086
0.0769 to 1.8311
0.2256
CTD screen
1.1017
0.0306
1.0374 to 1.1699
0.0016
IIF
4.2961
0.2977
2.3972 to 7.6993
<0.0001
EliA ENA
2.8036
0.3589
1.3875 to 5.6653
0.0041
LIA
7.6024
0.3342
3.9493 to 14.6345
<0.0001
SLE ()
Age (years)
0.9680
0.0103
0.9485 to 0.9878
0.0017
Female sex
0.7809
1.1675
0.0792 to 7.6986
0.8323
CTD screen
1.1309
0.0336
1.0588 to 1.2080
0.0003
IIF
4.5804
0.3904
2.1309 to 9.8456
0.0001
EliA ENA
2.0365
0.4773
0.7991 to 5.1899
0.1362
LIA
8.7782
0.4639
3.5360 to 21.7918
<0.0001
SSc ()
Age (years)
0.9688
0.0143
0.9420 to 0.9963
0.0265
Female sex
0.0707
0.9631
0.0107 to 0.4669
0.0265
CTD screen
0.9501
0.0668
0.8336 to 1.0830
0.4435
IIF
7.6505
0.5496
2.6051 to 22.4672
0.0002
EliA ENA
1.8686
0.6595
0.5130 to 6.8058
0.3431
LIA
4.7949
0.5912
1.5049 to 15.2774
0.0080
SS ()
Age (years)
1.0273
0.0163
0.9950 to 1.0606
0.0985
CTD screen
1.0595
0.0489
0.9626 to 1.1661
0.2374
IIF
1.8878
0.6031
0.5789 to 6.1559
0.2920
EliA ENA
12.7179
0.8660
2.3295 to 69.4327
0.0033
LIA
22.8184
1.1071
2.6055 to 199.8360
0.0047
MCTD ()
Age (years)
0.9947
0.0277
0.9421 to 1.0502
0.8466
CTD screen
1.0226
0.0629
0.9039 to 1.1569
0.7221
IIF
8.4246
1.2892
0.6733 to 105.4167
0.0983
EliA ENA
7.6465
1.3879
0.5036 to 116.1116
0.1427
LIA
3.5924
1.3567
0.2515 to 51.3162
0.3459
OR: odds ratio; SE: standard error; CI: confidence interval; IIF: indirect immunofluorescence; ENA: extractable nuclear antigen; LIA: line immunoassay; AARD: antinuclear antibody-associated rheumatoid disease; SLE: systemic lupus erythematosus; SSc: systemic sclerosis; SS: Sjögren’s syndrome; MCTD: mixed connective tissue disease. Multivariate analysis was performed using the presence of each AARD as a binary dependent variable and with the age, patient sex, and the results of CTD screen, IIF, EliA ENA, and LIA tests as covariates. The SS group and MCTD group were composed of females only.
