A Dual-Type L2 11-88 Peptide from HPV Types 16/18 Formulated in Montanide ISA 720 Induced Strong and Balanced Th1/Th2 Immune Responses, Associated with High Titers of Broad Spectrum Cross-Reactive Antibodies in Vaccinated Mice

Figure 1

(a) Schematic diagram of the recombinant pET-28a plasmids encoding dual-type fusion peptides. (b) Schematic representation of recombinant pET-HPV 16, pET-HPV 18, pET-HPV 31, and pET-HPV 45 plasmids harboring L2 11-200 from HPV types 16, 18, 31, and 45, respectively. (c) Purification of L2 dual-type fusion peptides and L2 proteins amino acids 11-200. The expressed proteins were purified by Ni-NTA affinity chromatography and purified polypeptides were visualized in Coomassie-stained gels. Lanes in (a): 1: molecular weight marker; 2, 3, and 4: purified DT 11-88 × 1, 69-81 × 3, and 17-36 × 3 fusion peptides, respectively. Lanes in (b): 1: molecular weight marker; 2, 3, 4, and 5: purified L2 11-200 proteins from HPV 18, 16, 31, and 45, respectively.

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