A Dual-Type L2 11-88 Peptide from HPV Types 16/18 Formulated in Montanide ISA 720 Induced Strong and Balanced Th1/Th2 Immune Responses, Associated with High Titers of Broad Spectrum Cross-Reactive Antibodies in Vaccinated Mice

Figure 2

Cross-reactive antibody responses that elicited in mice immunized with the dual-type L2 11-88 × 1 peptide emulsified in different adjuvants. Mice immunized (s.c.) three times at two-week interval with 25 μg of L2 11-88 × 1 formulated in alum (50 μg), CFA (at 1 : 1 ratio), Montanide ISA 720 (adjuvant/antigen ratio of 2 : 1), Montanide 50V2 (at 1 : 1 ratio), and MF59 (at 1 : 1 ratio). Mice administered with PBS were regarded as negative control. Two weeks after the last vaccination, blood samples were collected, and after preparation of twofold serially diluted sera from 1 : 100, cross-reactive antibody (IgG) titers were detected by end point dilution ELISA (a). In addition, IgG isotypes IgG1 (b), IgG2a (c), and IgG1/IgG2a ratio (d) were measured at a dilution (1 : 4000). Data are represented as means ± standard error of means (SEM) of triplicate from 5 mice per group. , , , and .

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